Invented by Petra Peters-Wendisch, Volker Wendisch, Nadja Alina HENKE, Universitaet Bielefeld

The market for Carotenoid biosynthesis and amino acid biosynthesis using corynebacterium recombinant is experiencing significant growth due to the increasing demand for natural and sustainable alternatives in various industries. Carotenoids are natural pigments found in plants and microorganisms that have numerous health benefits, including antioxidant and anti-inflammatory properties. Amino acids, on the other hand, are essential building blocks of proteins and play a crucial role in various biological processes. Corynebacterium is a genus of bacteria that has been widely used in biotechnology for the production of various compounds. Through genetic engineering techniques, scientists have successfully engineered corynebacterium strains to produce carotenoids and amino acids in large quantities. This recombinant approach allows for the efficient and cost-effective production of these valuable compounds. One of the key drivers of the market for carotenoid biosynthesis using corynebacterium recombinant is the growing consumer demand for natural food colorants. Carotenoids are widely used as natural colorants in the food and beverage industry, replacing synthetic dyes that are known to have adverse health effects. The use of corynebacterium recombinant enables the production of carotenoids in a sustainable and environmentally friendly manner, meeting the increasing demand for clean-label and natural ingredients. Furthermore, carotenoids have gained significant attention in the nutraceutical and pharmaceutical industries due to their potential health benefits. These compounds have been associated with the prevention of chronic diseases, such as cancer and cardiovascular diseases, as well as improved eye health and immune function. The ability to produce carotenoids using corynebacterium recombinant opens up new opportunities for the development of functional foods, dietary supplements, and pharmaceutical products. Similarly, the market for amino acid biosynthesis using corynebacterium recombinant is also witnessing substantial growth. Amino acids are essential for the production of proteins, which are crucial for various applications, including animal feed, biopharmaceuticals, and industrial enzymes. The recombinant production of amino acids using corynebacterium strains offers several advantages, such as high yields, scalability, and cost-effectiveness. The animal feed industry is one of the major consumers of amino acids, particularly lysine and methionine. These amino acids are essential for the growth and development of livestock and poultry. The use of corynebacterium recombinant allows for the production of amino acids in large quantities, meeting the increasing demand for high-quality and sustainable animal feed. In addition to animal feed, amino acids are also used in the production of biopharmaceuticals, such as antibodies and vaccines. The recombinant production of amino acids using corynebacterium strains offers a reliable and efficient method for the production of these complex molecules. This technology has the potential to revolutionize the biopharmaceutical industry by enabling the cost-effective production of life-saving drugs. Overall, the market for carotenoid biosynthesis and amino acid biosynthesis using corynebacterium recombinant is poised for significant growth in the coming years. The increasing demand for natural and sustainable alternatives in various industries, coupled with the advancements in genetic engineering techniques, will drive the adoption of this technology. The production of carotenoids and amino acids using corynebacterium recombinant offers numerous benefits, including improved product quality, reduced environmental impact, and cost-effectiveness. As a result, this market presents lucrative opportunities for companies operating in the biotechnology and food industries.

The Universitaet Bielefeld invention works as follows

The present invention is a method for producing astaxanthin in recombinant Gram-positive bacteria that contains a sequence of nucleic acids encoding a CrtZ-protein from F. Pelagi, and also a sequence of nucleic acids encoding a CrtW-protein.

Background for Carotenoid biosynthesis and amino acid biosynthesis using corynebacterium recombinant

Definitions

EDTA Ethylenediaminetetraacetate

EMB Ethambutol

t Time

UV Ultraviolet

Vis Visible

FIGURES

is SEQ NO. “is SEQ ID NO.

EXAMPLES

Example 1 : Co-Production Astaxanthine & Lysine

Bioinformatic tools: Clone Manager version 9.0 (SciEd)

Oligonucleotides:

Biological Material:

Cultivation:

Plasmid Isolation:

Colony-PCR:

Gel Electrophoresis:

PCR Clean-Up:

Conjugation:

Carotenoid Extraction:

Strain Construction By Conjugation And Transformation:

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