Invented by Mark Tarnopolsky, EXERKINE Corp

Exosomes are small extracellular vesicles that are secreted by cells and play a crucial role in intercellular communication. These vesicles contain a variety of biomolecules such as proteins, lipids, and nucleic acids, which can be transferred to recipient cells and modulate their behavior. In recent years, exosomes have emerged as promising therapeutic agents for the treatment of various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. The market for exosomes, also known as exersomes, is rapidly growing, and new methods of production and use are being developed to meet the increasing demand. Methods of Production Exosomes can be isolated from various sources, including cell culture supernatants, blood, and other bodily fluids. The most common method of exosome isolation is ultracentrifugation, which involves spinning the sample at high speeds to separate the exosomes from other cellular debris. However, this method is time-consuming and requires specialized equipment, making it unsuitable for large-scale production. Alternative methods of exosome production are being developed, including microfluidics-based approaches, which use microchannels to isolate and purify exosomes from complex biological samples. Another promising method is the use of genetically modified cells that overexpress exosomes, allowing for the production of large quantities of exosomes in a controlled manner. Use of Exersomes Exosomes have a wide range of potential therapeutic applications, including drug delivery, immunotherapy, and regenerative medicine. One of the most promising areas of exosome research is in cancer treatment, where exosomes can be used to deliver drugs directly to tumor cells or to stimulate the immune system to attack cancer cells. Exosomes are also being investigated for their potential in regenerative medicine, where they can be used to promote tissue repair and regeneration. For example, exosomes derived from mesenchymal stem cells have been shown to promote the growth of new blood vessels and improve tissue healing in animal models. In addition to their therapeutic potential, exosomes are also being used as biomarkers for disease diagnosis and monitoring. Exosome-based diagnostic tests are being developed for a range of diseases, including cancer, Alzheimer’s disease, and infectious diseases. Market Outlook The market for exosomes is expected to grow significantly in the coming years, driven by the increasing demand for novel therapeutics and diagnostic tools. According to a report by Grand View Research, the global exosome market size was valued at $102.9 million in 2020 and is expected to reach $2.28 billion by 2028, growing at a CAGR of 39.2% during the forecast period. The development of new methods of exosome production and use is expected to drive this growth, as researchers and companies seek to capitalize on the potential of these versatile biomolecules. As the market for exosomes continues to expand, it is likely that we will see new applications and innovations emerge, further fueling the growth of this exciting field.

The EXERKINE Corp invention works as follows

An exosome solution or pellet containing resuspended esosomes” is provided. The exosomes contain one or more metabolic compounds and are free of unwanted particles with a diameter greater than or less than 140 nm. The exosomes can be used to increase thermogenesis of white subcutaneous adipose tissues, induce mitochondrial biogenesis and/or mediate systemic effects from exercise in mammals.

Background for Exersomes: methods of production and use

Example 1: Isolation of exosomes

Example 2: Exosome Isolation using PEG-based Method

Example 3? Effect of Exercise on the Exosomes

Example 4″?Effects of exosomes on dermal fibroblasts

Example 5″?Effects of Exosome Administration

Example 6?” Effect of Exersomes on Type 2 Diabetes/Obesity Induced by Diet

Example 7. Effect of exersomes on Mitochondrial disease and Aging

Breeding of PolG Mutator Mouse with Littermate Wildtype mice

Endurance Exercise Protocol For PolG Mutator Mice

Administration of Exosomes

Example 8?” Effect of exersomes on Mitochondrial Biogenesis

Example 9.Effect of exersomes on Beige fat Gene Expression

Example 10?Proteomics & RNA-Sea Analysis of Exersomes”.

Example 11, Activity of METRNL

Example 12?” Activity of FNDC5

Details of the methods used in the examples

Acute Endurance Exercise Protocol For Wildtype Mice

Total DNA isolation

Oxygen Consumption Rate

MTT Assay Cell Density”.

Voluntary Activities Analyses

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