Invented by Zach Serber, Erik Jedediah Dean, Shawn Manchester, Katherine Gora, Michael Flashman, Erin Shellman, Aaron Kimball, Shawn Szyjka, Barbara Frewen, Thomas Treynor, Kenneth S. Bruno, Zymergen Inc

Microbial strain improvements have become increasingly important in various industries such as agriculture, food, and pharmaceuticals. The use of microbial strains has been a significant factor in the production of various products, including antibiotics, enzymes, and biofuels. However, the traditional methods of improving microbial strains have limitations, and the need for more efficient and precise methods has emerged. The development of a high-throughput (HTP) genome engineering platform has revolutionized the microbial strain improvement market, making it more efficient and cost-effective. The HTP genome engineering platform is a technology that enables the modification of microbial genomes at a high throughput rate. The technology uses a combination of synthetic biology, bioinformatics, and genetic engineering to modify microbial genomes. The platform has been used to improve microbial strains by introducing beneficial traits such as increased productivity, resistance to stress, and enhanced metabolic pathways. The market for microbial strain improvements by the HTP genome engineering platform has been growing rapidly. The market is driven by the increasing demand for microbial strains in various industries and the need for more efficient and precise methods of improving microbial strains. The market is also driven by the increasing investment in research and development of the HTP genome engineering platform. The pharmaceutical industry is one of the major industries that have benefited from the HTP genome engineering platform. The platform has been used to improve microbial strains that produce antibiotics, enzymes, and other pharmaceutical products. The platform has enabled the production of new antibiotics that are more effective against drug-resistant bacteria. The platform has also enabled the production of enzymes that are used in the production of various pharmaceutical products. The food industry is another industry that has benefited from the HTP genome engineering platform. The platform has been used to improve microbial strains that produce food additives, enzymes, and other food products. The platform has enabled the production of food additives that are more effective and safe for human consumption. The platform has also enabled the production of enzymes that are used in the production of various food products. The agricultural industry is also a major beneficiary of the HTP genome engineering platform. The platform has been used to improve microbial strains that are used in agriculture. The platform has enabled the production of microbial strains that are more resistant to stress and can survive in harsh environments. The platform has also enabled the production of microbial strains that can improve soil fertility and plant growth. In conclusion, the market for microbial strain improvements by the HTP genome engineering platform has been growing rapidly. The platform has revolutionized the microbial strain improvement market, making it more efficient and cost-effective. The market is driven by the increasing demand for microbial strains in various industries and the need for more efficient and precise methods of improving microbial strains. The HTP genome engineering platform has enabled the production of microbial strains that are more effective, safe, and can survive in harsh environments. The market is expected to continue growing as more industries adopt the HTP genome engineering platform.

The Zymergen Inc invention works as follows

The present disclosure presents a HTP microbial genome engineering platform which is computationally driven, integrates molecular biological, automation and advanced machine-learning protocols. This platform integrates HTP molecular tools to create HTP design libraries. These are derived, among other things, from scientific insight and iterative patterns recognition. The HTP genome engineering platform described in this document is strain-host agnostic, and can therefore be used across taxa. The disclosed platform may also be used to modulate any parameter of a microbial host that is of interest.

Background for Microbial strain improvements by a htp genome engineering platform

Definitions

Traditional Methods of Strain Improvement

Organisms that are amenable to genetic design

Generating Genetic Diversity Pools to be Used in the Genetic Design & HTP Microbial Engineering Platform”.

Product Recovery and Quantification”.

Computational analysis and prediction of effects of genome-wide genetic design criteria

Genomic Design and Engineering

Genomic Automation

HTP Microbial Strain Design Based on Genetic Design Predictions – An Example Workflow”.

EXAMPLES

Example 2: SNP Library Creation & HTP Transformation

Cloning Transformation Vectors

D. Looping out Selection Markers

E. Summary

Example 2: HTP Genomic Engineering?Implementation of a SNP Library to Rehabilitate/Improve an Industrial Microbial Strain

A. “A. Identification of SNPs within Diversity Pool

B. SNP Swapping Analysis

C. Use of Epistatic Mapping for Determining Beneficial SNP Compounds

Example 3: HTP Genomic Engineering?Implementation of a SNP Swap Library to Improve Strain Performance in Lysine Production in Corynebacterium

C. Tank Culture validation

Example 4: HTP Genomic Engineering?Implementation of a Promoter Swap Library to Improve an Industrial Microbial Strain

Click here to view the patent on Google Patents.