Invented by Randy Deinhammer, Suzanne Clark, Mauricio Quiros, John Matthews, Anne Glud Hjulmand, Chee Leong Soong, Tomoko Matsui, Shinobu Takagi, Novozymes AS

The market for processes of producing ethanol has experienced significant growth in recent years, driven by increasing demand for renewable energy sources and the need to reduce greenhouse gas emissions. Ethanol, a type of biofuel made from renewable resources such as corn, sugarcane, or cellulosic materials, is considered a viable alternative to fossil fuels due to its lower carbon footprint. One of the key factors driving the market for ethanol production processes is the growing awareness of the environmental impact of traditional fossil fuels. Ethanol is a cleaner-burning fuel that produces fewer emissions compared to gasoline or diesel. As governments and industries worldwide strive to reduce their carbon footprint and comply with stricter environmental regulations, the demand for ethanol as a fuel additive or standalone fuel has surged. Another factor contributing to the market growth is the increasing focus on energy security and independence. Ethanol can be produced domestically, reducing reliance on imported fossil fuels and enhancing energy self-sufficiency. This aspect has particularly gained traction in countries with limited oil reserves or those looking to diversify their energy sources. The market for ethanol production processes is also driven by government policies and incentives. Many countries have implemented renewable fuel standards or biofuel blending mandates, requiring a certain percentage of ethanol to be mixed with gasoline or diesel. These policies create a stable market demand for ethanol, encouraging investments in production processes and technologies. In terms of production processes, there are several methods used to produce ethanol, each with its own advantages and limitations. The most common process is fermentation, where sugars from feedstocks such as corn or sugarcane are converted into ethanol by yeast. This method is widely used and relatively cost-effective, making it the dominant process in the market. However, advancements in technology have led to the development of alternative processes such as cellulosic ethanol production. This method utilizes non-food feedstocks like agricultural residues, wood chips, or dedicated energy crops, making it a more sustainable and environmentally friendly option. Although cellulosic ethanol production processes are still in the early stages of commercialization, they hold great potential for the future of ethanol production. The market for ethanol production processes is highly competitive, with numerous companies and research institutions investing in research and development to improve efficiency, reduce costs, and enhance the sustainability of ethanol production. Technological advancements, such as the use of genetically modified organisms or enzymes to enhance fermentation efficiency, are constantly being explored to make ethanol production more economically viable. In conclusion, the market for processes of producing ethanol is experiencing significant growth due to increasing demand for renewable energy sources, the need to reduce greenhouse gas emissions, and government policies promoting the use of biofuels. As the world transitions towards a more sustainable energy future, ethanol production processes will continue to evolve, offering new opportunities for investors, researchers, and industry players.

The Novozymes AS invention works as follows

The present invention is directed to methods for producing fermentation products using starch-containing materials, in which a thermostable beta-amylase or optionally a protease thermostable are present and/or included during liquefaction. The invention also relates a composition that can be used in a method of the invention.

Background for Processes of producing ethanol

Saccharification & Fermentation

Fermentation Medium

Fermenting Organisms

Starch-Containing Materials

Fermentation Products



Maltogenic Amylase

Alpha-Amylase & Protease Composition

Use of the Composition of the Invention

Materials & Methods




Protease Assays

AZCL-Casein Assay

Glucoamylase activity (AGU)

Alpha-Amylase Activity” (KNU).

Determination Pullulanase Activity


Example 1

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